Journal: BMC Genomics

Article Title: Sex specific expression and distribution of small RNAs in papaya

doi: 10.1186/1471-2164-15-20

Figure Lengend Snippet: Size distribution of sRNA reads from three libraries. Unique sequences were obtained from each library after adapter trimming and removing other known classes of contaminant RNAs.

Article Snippet: The band corresponding to the RNA standard of 18–30 nucleotides was then excised from the gel and eluted overnight in 0.4 M NaCl at 4°C, and subsequently ligated with 5’ and 3’ Illumina small RNA adapters.

Techniques:

Journal: BMC Genomics

Article Title: Sex specific expression and distribution of small RNAs in papaya

doi: 10.1186/1471-2164-15-20

Figure Lengend Snippet: Map of the sRNA alignment on the sex specific regions of the papaya sex chromosomes. HSY- hermaphrodite specific region of the Yh chromosome, MSY male specific region of the Y chromosome, X – corresponding region of the X chromosome. The yellow lines indicate the gap on the physical map of the respective chromosome. The x-axis represents the chromosomes and the y-axis represents the sRNA alignment density on the respective chromosome.

Article Snippet: The band corresponding to the RNA standard of 18–30 nucleotides was then excised from the gel and eluted overnight in 0.4 M NaCl at 4°C, and subsequently ligated with 5’ and 3’ Illumina small RNA adapters.

Techniques:

Journal: BMC Genomics

Article Title: Sex specific expression and distribution of small RNAs in papaya

doi: 10.1186/1471-2164-15-20

Figure Lengend Snippet: Diagrammatic representation of papaya sex chromosomes showing putative centromeres and inversion region. The sex specific regions are shown on dark blue and pseudo-autosomal regions are shown on light blue. Bottom panel shows the zoomed view of sex determining region. The blue dotted line represents the gap on physical map with the putative centromere represented by dotted circle. The red curves indicate the higher sRNA expressing loci adjacent to the gaps; the purple bar below X chromosome represents an X specific genes. The green dotted lines indicate approximate position for the inversion I [30] on the Y chromosome relative to the X chromosome.

Article Snippet: The band corresponding to the RNA standard of 18–30 nucleotides was then excised from the gel and eluted overnight in 0.4 M NaCl at 4°C, and subsequently ligated with 5’ and 3’ Illumina small RNA adapters.

Techniques: Expressing